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NGF-β human
NGF-β human from MilliporeSigma
Be the first to write a review!
Citations:
(14)
Supplier Page
Supplier Page from
MilliporeSigma for
NGF-β human
Description
from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NGF-β human
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
N1408
Species
human
Molecule Name
HTR3C, HTR3B, HTR5B
NCBI Full Gene Name
5-hydroxytryptamine receptor 3B, 5-hydroxytryptamine receptor 3C, 5-hydroxytryptamine receptor 5B, pseudogene
NCBI Gene Aliases
5-HT5B, HTR5B, GPR134, 5-HT3B
Applications
mammalian cell culture
Format
Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin
Quantity
0.1MG, 5X0.1MG
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MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (14)
(1)
Stressed neuronal cells can recover from profound membrane blebbing, nuclear condensation and mitochondrial fragmentation, but not from cytochrome c release
Scientific reports
July 8, 2023
You W, Zhou T, Knoops K, Berendschot TTJM, van Zandvoort MAMJ, Germeraad WTV, Benedikter B, Webers CAB, Reutelingsperger CPM, Gorgels TGMF.
R&D Systems
) coated
cell culture
plate in low serum conditions (1%
horse
serum) and with 50 ng/ml of NGF (NGF;
N1408
,
Sigma-Aldrich
) for 6–7 days. All cells were cultured at 37 °C in a 5% CO2 incubator. SH-SY5Y and
HeLa cells
(2)
Gardenia jasminoides Extract, with a Melatonin-Like Activity, Protects against Digital Stress and Reverses Signs of Aging
International Journal of Molecular Sciences
March 3, 2023
Morgane De Tollenaere, Emilie Chapuis, Jessy Martinez, Chantal Paulus, Joran Dupont, Eglantine Don Simoni, Patrick Robe, Bénédicte Sennelier-Portet, Daniel Auriol, Amandine Scandolera,
et al.
Fisher,
450-10
, batch H170806), NT3 at 10 ng/mL (
PeproTech
450-03
, batch H171010), NGF at 10 ng/mL (
Sigma
N1408
. batch SLBW7063) and 1% PS antibiotics. Cells were maintained in culture at 37 °C under 5% CO2. The
(3)
Sex-Related Pain Behavioral Differences following Unilateral NGF Injections in a Rat Model of Low Back Pain
Biology (Basel)
June 16, 2022
Michael Syrett, Nicholas R. Reed, William R. Reed, Madison L. Richey, Andrey Frolov, Joshua W. Little
were performed under isoflurane anesthesia [28,32].
Human
recombinant NGF (
Sigma-Aldrich
catalog #
N1408
) was dissolved in 50 μL of phosphate-buffered saline (PBS) at 0.8 μM and a pH of 7.2–7.3. Control
rats
(4)
Single Cell Analysis of Reversibility of the Cell Death Program in Ethanol-Treated Neuronal PC12 Cells
International journal of molecular sciences
February 28, 2022
You W, Berendschot TTJM, Knoops K, van Zandvoort MAMJ, Webers CAB, Reutelingsperger CPM, Gorgels TGMF.
coated
cell culture
plate in low serum conditions (1%
horse
serum, no FBS) and 50 ng/mL of NGF (NGF;
N1408
,
Sigma-Aldrich
). Mature neuronal
PC12 cells
were defined as cells harboring
neurites
at least two
cell body
(5)
Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus
Science advances
January 7, 2022
Scaramuzzino C, Cuoc EC, Pla P, Humbert S, Saudou F.
(antisense)]. Reagents Human BDNF (50 ng/ml) (
PeproTech
,
450-20
), human NGF-β (50 ng/ml) (
Sigma-Aldrich
,
N1408
), and human EGF (2.5 ng/ml) (
Sigma-Aldrich
,
E9644
) were resuspended in PBS containing bovine serum albumin
(6)
Role of the Sec22b–E-Syt complex in neurite growth and ramification
Journal of Cell Science
September 15, 2020
Alessandra Gallo, Lydia Danglot, Francesca Giordano, Bailey Hewlett, Thomas Binz, Christian Vannier, Thierry Galli
1 µg/ml collagen (
Sigma
C7661
) solution. Then, cells were differentiated with hNGF-α (
Sigma-Aldrich
N1408
) at 50 ng/ml for 3–4 days. PC12
transfections
were carried out with an Amaxa Nucleofection Kit V (
Lonza
(7)
Temporal perturbation of ERK dynamics reveals network architecture of FGF2/MAPK signaling
Molecular Systems Biology
November 19, 2019
Yannick Blum, Jan Mikelson, Maciej Dobrzyński, Hyunryul Ryu, Marc‐Antoine Jacques, Noo Li Jeon, Mustafa Khammash, Olivier Pertz
and other reagents","Chemicals, enzymes, and other reagents"
hEGF
,
Sigma
,
E9644
NGF‐beta
human
,
Sigma
,
N1408
hBFGF
,
Sigma
,
F0291
Dextran Alexa 546,
Thermo Fisher Scientific
,
D22911
NaClO3,Sigma,403016 DAPI,Sigma,D9542 Software
(8)
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of
Salmonella
spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
International journal of molecular sciences
August 28, 2018
Mikołajczyk A, Kozłowska A, Gonkowski S.
glass. Cultures were cultivated in TNB medium 1 mL per well with 5 ng/mL nerve growth factor beta (NGF;
N1408
;
Sigma Aldrich
; Berlin, Germany) to maintain the survival of
neurons
[81]. After 36 h
incubation
at
(9)
Nerve Growth Factor Stimulates Glioblastoma Proliferation through Notch1 Receptor Signaling
Journal of Korean Neurosurgical Society
July 1, 2018
Jun Chul Park, In Bok Chang, Jun Hyong Ahn, Ji Hee Kim, Joon Ho Song, Seung Myung Moon, Young-Han Park
medium. After 24 hours
incubation
at 37°C, cells were treated for 48 hours with drugs (human NGF-β) (
N1408
,
Sigma-Aldrich
, St. Louis, MO, Nerve Growth Factor Effects in
Glioblastoma
via
Notch1
| Park JC,
(10)
Growth factor choice is critical for successful functionalization of nanoparticles
Frontiers in Neuroscience
September 2, 2015
Josephine Pinkernelle, Vittoria Raffa, Maria P. Calatayud, Gerado F. Goya, Cristina Riggio, Gerburg Keilhoff
2 µg/mg of NPs (Calatayud et al., 2013). Preparation of NGF-PEI-NPs and NGF:PEI-NPs NGF-β (
Sigma
,
N1408
) was marked with a fluorescent tag by
labeling
a protein mixture of NGF/BSA (
1:6
w/w) with Alexa Fluor
(11)
Differential regulation of macrophage phenotype by mature and pro-nerve growth factor
Journal of neuroimmunology
August 15, 2015
Williams KS, Killebrew DA, Clary GP, Seawell JA, Meeker RB.
for 1 or 24 hours using three different conditions: NGF human recombinant protein (100 ng/mL,
Sigma
N1408
), proNGF human recombinant protein targeted to high affinity sites (1 ng/ml, Alamone N-280), or vehicle.
(12)
A monoclonal antibody TrkB receptor agonist as a potential therapeutic for Huntington's disease
PloS one
January 1, 2014
Todd D, Gowers I, Dowler SJ, Wall MD, McAllister G, Fischer DF, Dijkstra S, Fratantoni SA, van de Bospoort R, Veenman-Koepke J,
et al
.
Pen/strep. Reagents BDNF (
PHC7074
) and ToxBLAzer reagent (K1136,
P36400
) were obtained from
Life Technologies
. NGF-β (
N1408
), NT-3 (
N1905
), NT-4 (
N1780
), and
Thapsigargin
(
T9033
) were obtained from
Sigma-Aldrich
. Tool small
(13)
Role of neurotrophins on postnatal neurogenesis in the thalamus: prenatal exposure to ethanol
Neuroscience
April 14, 2011
Sandra M. Mooney, Michael W. Miller
for a short (2 hr) or long (24 hr) time. Other slices were treated with NGF (0, 20, 40, or 80 ng/ml;
N1408
; St Louis MO) or with BDNF (50 ng/ml) and NGF (0, 20, 40, or 80 ng/ml) for 4 hr. Three or four slices
(14)
Combination of Polymer Technology and Carbon Nanotube Array for the Development of an Effective Drug Delivery System at Cellular Level
Nanoscale Research Letters
July 1, 2009
Cristina Riggio, Gianni Ciofani, Vittoria Raffa, Alfred Cuschieri, Silvestro Micera
200 μg/mL. BSA was used as “protein model”, as its molecular weight is similar to that one of NGF (
N1408
from
Sigma
, reconstituted in a 0.1% BSA solution in PBS) and its concentration can be much more easily
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NGF-β human from MilliporeSigma
Supplier Page
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